polyjet (signagen laboratories, sl100688) transfection reagent  (SignaGen)

Journal: iScience

Article Title: Designer polyQ fusion proteins sequester USP7/HDM2 for modulating P53 functionality

doi: 10.1016/j.isci.2025.112025

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Article Snippet: PolyJet reagent , SignaGen , Cat# SL100688.

Techniques: Virus, Recombinant, Protease Inhibitor, Reverse Transcription, SYBR Green Assay, Sequencing, Software

Journal: Cell Genomics

Article Title: Type 1 diabetes risk genes mediate pancreatic beta cell survival in response to proinflammatory cytokines

doi: 10.1016/j.xgen.2022.100214

Figure Lengend Snippet: Identifying transcriptional regulators affecting T1D risk in beta cell cCREs with SNP-SELEX (A) Design of HT-SELEX-seq experiment. (B) Top: Example of enrichment profiles of bound oligos within an experiment and of an SNP with preferential binding. Bottom: Distribution of the number of variants with allelic binding per TF across 489 TFs and table summarizing the number of bound variants and allelic binding variants across TFs. (C) Enrichment of variants with allelic binding for T1D association among all tested variants, variants in beta cell cCREs, and variants in cytokine-responsive beta cell cCREs. Values are odds ratios and error bars are 95% CI from Fisher’s exact test. (D) Enrichment of variants with allelic binding of specific TF sub-families for T1D association among variants in cytokine-responsive beta cell cCREs. Values represent odds ratios by Fisher’s exact test, and points are colored by p value. (E) Regional plot of T1D association, with variants with p < 10 −4 in black; bulk ATAC-seq from human islets. Treatment abbreviations as in Figure 1 . (F) EMSA using nuclear extract (NE) from cytokine-treated MIN6 with probes for each allele of rs10483809. (G) Luciferase assays for rs10483809 alleles in MIN6 in untreated or high-dose cytokines compared with empty vector. Values are mean and error bars SD from n = 9 transfections, with uncorrected p values shown from two-sided t tests.

Article Snippet: To package lentivirus encoding the human GeCKO-V2 CRISPR screen library, plasmids containing the gRNA library (Addgene, 1000000048) were transfected into the HEK 293 T cells together with the lentiviral packing vectors, pMD2.G (Addgene, 12259) and psPAX2 (Addgene, 12260), using a PolyJet TM DNA transfection reagent (Signagen Laboratories, 504788).

Techniques: Binding Assay, Luciferase, Plasmid Preparation, Transfection